Composite

Part:BBa_K3813000:Design

Designed by: Matthew Kaung   Group: iGEM21_ASIJ_Tokyo   (2021-07-28)


Mucin(MUC 1) with optimized TA promoter and Lux terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1913
    Illegal NgoMIV site found at 1973
    Illegal NgoMIV site found at 2032
    Illegal NgoMIV site found at 2087
    Illegal NgoMIV site found at 2122
    Illegal NgoMIV site found at 2182
    Illegal NgoMIV site found at 2362
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is part of the 9 constructs we made for optimal production of our biomarker of interest. This part was successful but protein concentration was relatively low.


Source

The source of the Mucin 1 sequence was obtained from the "Homo sapiens chromosome 1, GRCh38.p13 Primary Assembly" sequence from NCBI.

References

[1]Homo sapiens chromosome 1, GRCh38.p13 Primary Assembly - Nucleotide - NCBI. (n.d.). Retrieved August 2, 2021, from https://www.ncbi.nlm.nih.gov/nuccore/NC_000001.11?report=fasta&from=155185824&to=155192915&strand=true